红花种子脂肪酸代谢关键基因的挖掘与分析OA

[Objective]To identify the key genes affecting fatty acid development in safflower seeds,so as to provide a theoretical basis and technical support for a deeper understanding of the molecular mechanisms of safflower fatty acid synthesis.[Method]Safflower seeds collected at 4,10,16,and 22 days after flowering(referred to as D4,D10,D16,and D22,respectively)were used as materials.The contents of 37 fatty acids in seeds at different developmental stages were measured.Transcriptome sequencing and bioinformatics analysis methods were employed to identify differentially expressed genes.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment analy-ses were conducted to explore the biological processes and biochemical metabolic pathways in which the differentially expressed genes in safflower seeds at different growth stages were involved.[Result]n the early stage of safflower seed development,saturated fatty acids accounted for 56.52%of the total fatty acids.After the 10th day after flowering,the content of linoleic acid increased from 5.03%to 75.41%.KEGG analysis indicated that the period from D4 to D10 was the critical stage for safflower fatty acid synthesis,with 37 differentially expressed genes enriched in the biosynthetic pathway of unsaturated fatty acids.The encoded products included stearoyl-CoA desaturase,omega-6 fatty acid desaturase,very-long-chain enoyl-CoA reductase,and acetyl-CoA acyltransferase.[Conclusion]A total of 29 genes involved in the fatty acid metabolic process of safflower seeds were identified.It was found that the expression of ACC,FATA,DGAT1 and FAD2 enzyme genes promoted the accumulation of fatty acids in safflower seeds.