积雪草酸对乙型肝炎大鼠的干预效果及对肝组织 NF-κB/STAT3/ERK 通路的影响OA
Intervention effect of asiatic acid on rats with hepatitis B and its influence on NF-κB/STAT3/ERK signaling pathway in liver tissue
目的:探讨积雪草酸(AA)对乙型肝炎大鼠的干预效果及对肝组织 NF-κB/STAT3/ERK 通路的影响.方法:60 只大鼠随机分为正常对照组,模型组,AA 低、中、高剂量组(40、80、120 mg/kg),阳性对照组(恩替卡韦,0.5 mg/kg),每组10 只.除正常对照组外,余5 组一次性尾静脉注射1.7×109 拷贝数 rAAV-1.3HBV 建立乙型肝炎大鼠模型;之后正常对照组和模型组灌胃等体积生理盐水,余4 组灌胃给药,1 次/d,连续10 d.全自动生化分析仪检测血清 ALT、AST 水平;ELISA 法检测血清 HA、LN 和 HBsAg、HBeAg 水平;HE 染色观察肝组织病理学变化;RT-qPCR 法检测肝脏和血清 HBV DNA;Western blot 法检测肝组织中 NF-κB p65、STAT3、ERK 蛋白磷酸化水平.结果:模型组大鼠肝细胞排列紊乱、坏死肿胀,炎性细胞浸润明显;3 个 AA 组和阳性对照组肝细胞坏死减轻,细胞分布较均匀,炎性细胞浸润减少.模型组大鼠血清 ALT、AST、HA、LN 水平,肝脏 NF-κB p65、STAT3、ERK 蛋白磷酸化水平较正常对照组升高(P<0.05).与模型组比较,AA 中、高剂量组和阳性对照组上述指标及 HBV DNA 载量均下降(P<0.05);AA 高剂量组与阳性对照组差异无统计学意义(P>0.05).结论:AA 可以抑制 NF-κB/STAT3/ERK 信号通路,减少 HBV 复制,改善乙型肝炎大鼠的肝组织损伤.
Aim:To investigate the intervention effect of asiatic acid(AA)on the rats with hepatitis B and its influence on the NF-κB/STAT3/ERK signaling pathway in liver tissue.Methods:Sixty rats were randomly divided into normal control group,model group,low-,medium-,and high-dose AA groups(40,80,and 120 mg/kg),and positive control group(ente-cavir,0.5 mg/kg),with 10 rats in each group.Except for the normal control group,the other 5 groups were established as hepatitis B rat models by a single tail vein injection of 1.7×109 copy number rAAV-1.3HBV.Subsequently,the normal control group and the model group received intragastric administration of an equal volume of normal saline,while the other 4 groups received intragastric drug administration once daily for 10 days.Serum ALT and AST levels were detected using an automated biochemical analyzer;serum HA,LN,HBsAg,and HBeAg levels were measured by ELISA;HE staining was per-formed to observe hepatic histopathological changes;RT-qPCR was used to detect HBV DNA in the liver and serum sam-ples;and Western blot was employed to assess the phosphorylation levels of NF-κB p65,STAT3,and ERK proteins in liver tissue.Results:In the model group,hepatocytes showed disordered arrangement,swelling,necrosis,and significant inflam-matory cell infiltration.The three AA groups and the positive control group demonstrated reduced hepatocyte necrosis,gradu-ally uniform cell distribution,and decreased inflammatory cell infiltration.Serum ALT,AST,HA,and LN levels,as well as hepatic NF-κB p65,STAT3,and ERK protein phosphorylation levels in the model group were significantly elevated com-pared with the normal control group(P<0.05).Compared with the model group,the indicators mentioned above and HBV DNA load decreased in the medium-,high-dose AA groups and the positive control group(P<0.05),and no statistically significant difference was observed between the high-dose AA group and the positive control group(P>0.05).Conclu-sion:AA could inhibit HBV replication,and improve the liver tissue injury of rats with hepatitis B which may be associated with inhibiting the activation of NF-κB/STAT3/ERK signaling pathway.
陈茂丽;韩成美;陈龙
山东第一医科大学附属人民医院感染科 济南 271100山东第一医科大学附属人民医院感染科 济南 271100石家庄市人民医院重症医学科 石家庄 050000
医药卫生
积雪草酸乙型肝炎NF-κB/STAT3/ERK 信号通路大鼠
asiatic acidhepatitis BNF-κB/STAT3/ERK signal pathwayrat
《郑州大学学报（医学版）》 2026 (2)
36-40,5
山东中医药科技项目(2021M030)
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