小檗碱与表没食子儿茶素没食子酸酯自组装纳米体系对急性溃疡性结肠炎小鼠的干预效果OA

Aim:To evaluate the therapeutic effect of self-assembled nanocomposite(BE)of berberine(BBR)and epi-gallocatechin gallate(EGCG)on acute ulcerative colitis(UC)in mice.Methods:BE was prepared by nanoprecipitation,and the states of dissolution in water of BBR,EGCG and BE were observed.Thirty 6-week-old male C57BL/6J mice were randomly divided into 6 groups(5 in each group).Mice in the blank group were given ordinary drinking water and intragas-trically administered 100 μL distilled water daily.Mice in the model group,BBR group,EGCG group,B+E group and BE group were given dextran sulfate sodium solution,and were intragastrically administered 100 μL distilled water,40 mg/kg BBR,60 mg/kg EGCG,50 mg/kg physical mixture of BBR and EGCG,and 50 mg/kg BE solution daily,respectively,for 7 consecutive days.Mice were sacrificed on day 9,and colons were isolated.Disease activity index(DAI),colon length and spleen index were evaluated.Histopathological analysis was performed by HE and picrosirius red staining.The mRNA ex-pression levels of IL-6,TNF-α and IL-1β in colon tissues were detected by RT-qPCR.Results:BBR appeared as a yellow suspension in water,EGCG solution was colorless and transparent,and BE solution was a uniform pale yellow clear liquid without obvious precipitation or stratification.During the experiment,DAI of the blank group was 0.Mice in the other 5 groups showed body weight loss and increased DAI scores,and the changes in body weight and DAI in the BE group were smaller than those in the other 4 groups(P<0.05).Compared with the blank group,the model group showed shortened co-lon length,elevated percentage of fibrotic area,mRNA expression levels of IL-6,TNF-α and IL-1β,as well as spleen index(P<0.05).Compared with the model group,the above indicators and comprehensive pathological score in the BBR,EGCG,B+E and BE groups all showed a downward trend,and the changes were most significant in the BE group(P<0.05).Conclusion:BE has good water solubility,can effectively alleviate UC,and its efficacy is superior to BBR,EGCG a-lone and their physical combination.