猪流行性腹泻病毒CH/HN/2024分离鉴定及遗传进化分析OA

To clarify the genetic variation characteristics of Porcine epidemic diarrhea virus(PEDV),this study conducted detection and isolation of viruses from PEDV-positive samples,followed by identification via experiments including RT-PCR,indirect immunofluorescence assay(IFA)and transmission electron microscopy(TEM).Meanwhile,whole-genome sequencing and genetic evolution analysis were performed based on next-generation sequencing(NGS)technology.The results showed that one PEDV isolate was successfully obtained in this study,designated as PEDV CH/HN/2024 strain.Homology alignment analysis revealed that the complete of this isolate shared extremely high similarity with those of the ZQ2022 strain.Phylogenetic tree analysis indicated that the CH/HN/2024 strain clustered in the same branch as the ZQ2022 strain,belonging to the G2a subtype.Analysis of key antigenic epitope regions of the S protein demonstrated that,the S protein of this isolate exhibited 10 and 2 amino acid sequence variations in the COE and SS6 regions compared with the classic CV777 strain,respectively.This study intended to lay a data foundation for clarifying the current epidemic characteristics of PEDV and deeply deciphering the genetic evolution rules of the virus,and provide a scientific basis for subsequent PED vaccine research and development,thereby facilitating the improvement and optimization of the comprehensive prevention and control system for PED in the swine industry.