小麦bZIP基因家族鉴定及在叶夹角形成过程中的表达分析OA
Identification of the wheat bZIP gene family and expression analysis during leaf angle formation
叶夹角是决定作物株型与产量的关键农艺性状,挖掘其调控基因对作物株型改良具有重要意义.本研究基于小麦品种KN9204基因组,利用生物信息学方法鉴定到246个bZIP类转录因子,并对其理化性质、系统进化、染色体定位、保守基序和功能结构域等进行了系统分析;进一步结合转录组数据,分析了 bZIP基因家族在叶片不同组织和发育时期的表达模式.结果表明,多数bZIPs蛋白结构不稳定;保守基序分析显示,相近亚家族之间存在特定基序保守性;组织特异性表达分析共鉴定到41个差异表达bZIPs,其中9个在叶枕组织中显著上调.从中选取4个基因进行qRT-PCR验证,其表达趋势与转录组结果一致.本研究初步筛选到可能参与小麦叶枕发育和叶夹角形成的bZIPs候选基因,为解析叶枕发育和叶夹角形成调控机制提供了理论依据和遗传资源.
Leaf angle is a key agronomic trait that determines plant architecture and yield in crops.Mining regulatory genes underlying leaf angle is of great significance for improving plant architecture.Using the genome of wheat cultivar KN9204,this study identified 246 bZIP transcription factors via bioinformatics methods,and systematically analyzed their physicochemical properties,phylogenetic relationships,chromosomal locations,conserved motifs,and functional domains.Furthermore,transcriptome data were integrated to analyze the expression patterns of bZIP family genes in different tissues and developmental stages of leaves.The results revealed that most bZIP proteins exhibit structural instability.Conserved motif analysis indicated that closely related subfamilies share specific motif conservation.Tissue-specific expression profiling identified 41 differentially expressed bZIP members,among which 9 were significantly upregulated in the lamina joint.Four of these genes were selected for qRT-PCR validation,and their expression trends were consistent with the transcriptomic data.This study preliminarily screened potential bZIP candidate genes as candidates that may be involved in wheat lamina joint development and leaf angle formation,providing a theoretical foundation and genetic resources for deciphering the regulatory mechanisms underlying lamina joint development and leaf angle formation.
王一墨;王菁;谢登雷;张亚舟;李丽;刘籽彤;王荣纳
河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北保定,071001
农业科技
小麦bZIPs转录因子叶夹角表达模式分析
wheatbZIPs transcription factorleaf angledifferential expression analysis
《河北农业大学学报》 2026 (2)
31-38,8
河北省省级科技计划(24466301D)国家自然科学基金青年项目(32201706)河北省自然科学基金优秀青年科学基金项目(C2022204088)河北省青年人才项目(QN2024024).
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