基于树突状细胞介导的Th2免疫应答探讨平喘颗粒抑制哮喘气道炎症的机制OA

Objective:To investigate the effect of Pingchuan granules on dendritic cells(DCs)-mediated Th2 immune re-sponse and elucidate its mechanism in inhibiting airway inflammation in asthma.Methods:C57BL/6J mice were randomly divided into the control group,model group,Pingchuan granule group,and Budesonide group.Asthma models were established by sensiti-zation and challenge with ovalbumin(OVA).Airway inflammation and goblet cell hyperplasia were assessed using hematoxylin-eosin(H&E)staining and periodic acid-Schiff(PAS)staining,respectively.Levels of Th2 cytokines(IL-4,IL-5,IL-13)and OVA-specific IgE in bronchoalveolar lavage fluid(BALF),as well as the levels of chemokines(CCL17,CCL22)and FceRI in lung tissue,were measured by enzyme-linked immunosorbent assay(ELISA).Flow cytometry was employed to de-tect the expression of DCs maturation markers(CD80,CD86,MHC-Ⅱ)and the proportion of Th2 cells in lung tissues,draining lymph nodes,and mediastinal lymph nodes.Results:Compared to the model group,Pingchuan granules significantly reduced air-way inflammation scores and PAS scores(P<0.01).Furthermore,Pingchuan granules markedly inhibited BALF levels of IL-4,IL-5,IL-13,and OVA-specific IgE(P<0.01),and decreased the pulmonary contents of CCL17,CCL22,and FceRI(P<0.01).In addition,Pingchuan granules significantly suppressed the expression of CD80,CD86,and MHC-Ⅱ in DCs and reduced the proportion of Th2 cells in lung tissues,draining lymph nodes,and mediastinal lymph nodes when compared to the model group(P<0.01).Conclusion:Pingchuan granules can effectively alleviate airway inflammation in asthma,and its mechanism may be associated with the inhibition of DCs maturation and activation,thereby blocking the DCs-mediated Th2 immune response.