复方扶芳藤合剂对黑色素瘤小鼠脾脏CD4+T细胞的调节作用OA

Objective:To explore the regulatory effect of Compound Fufangfuteng Mixture(CFM)on CD4+T cells in the spleen of melanoma mice.Methods:Randomly divide 50 female C57BL/6 mice into 5 groups:blank group(Blank),tumor model group(B16),high-dose Compound Fufangfuteng Mixture group(CFM-H),medium-dose Compound Fufangfuteng Mixture group(CFM-M),and low-dose Compound Fufangfuteng Mixture group(CFM-L).Pre-treat the mice with pure water or different doses of CFM by gavage for 21 days,followed by subcutaneous injection of B16 cells to establish melanoma model.After 2 weeks,ter-minate the experiment,harvest the mouse spleens,and use flow cytometry to detect the proportion of T cells and their activation,proliferation,apoptosis,inhibition,transcription factor expression and cytokine secretion.Results:CFM had no significant effect on mouse body weight and thymus index,but the medium dose of CFM could increase the spleen index;the medium dose of CFM reduced the proportion of CD4+T cells and increased the proportion of CD8+T cells,with no significant effect on the total T cell proportion;the medium dose of CFM could promote the increase in the number of Th1 and Th2 cells;The medium dose of CFM could promote the expression of CD69 and CD38 on T cells;the high dose of CFM could promote the expression of CD95 on T cells,while the medium and low doses of CFM could promote the expression of PD-1 on T cells;The high dose of CFM could promote the secretion of IL-4,the medium dose of CFM could promote the increase of IL-2,IFN-γ,IL-4,and IL-10,and the low dose of CFM could promote the secretion of TNF-α and IL-4;both high and medium doses of CFM could increase the expres-sion of NF-κB,the medium dose of CFM could decrease the expression of T-bet and GATA-3,and the low dose of CFM could reduce the expression of T-bet.Conclusions:CFM can activate CD4+T cells and promote the differentiation of Th1 and Th2 cell subsets.