继代培养对马桑菌生长速度和漆酶活力的影响OA

To investigate the effects of subculture on the growth and laccase activity of Lentinula edodes(a characteristic edible fungus in Guizhou Province),the mycelial growth characteristics,antagonistic reactions,and laccase activity of the fungus during subculture were studied.The results showed that no antagonistic reaction was observed between any subcul-tured strain and the F1 generation,indicating that genetic variation within the F15 generation could not be determined based solely on phenotypic observation.The daily mycelial growth rate exhibited an overall decreasing trend with increasing sub-culture generations,with the F1 and F7 generations showing the fastest growth,reaching a daily growth rate of 0.95 mm/d.The mycelial density and whiteness near the inoculation point were higher in the F1 to F5 generations compared to other subcultured strains,whereas sectoring was observed in the F13 and F15 generations.Laccase activity was positively pro-moted from F1 to F9 generations,but fluctuated more irregularly after the F9 generation.The highest laccase activity(113.89 U/L)was observed in the F13 generation,which was 1.26 times higher than that of the F1 generation.Considering both mycelial growth and laccase activity,it is recommended that the number of subculture generations should be con-trolled within F5 in the practical spawn production of Lentinula edodes to ensure spawn quality and cultivation safety.