皇竹草间作白刺花对根际土壤细菌群落结构及多样性的影响OA

[Objective]The effects of Pennisetum sinese-Sophora viciifolia intercropping on soil bacterial community structure and diversity were studied to evaluate the potential improvement effect of this cropping system on soil microbial ecosystems and provide theoretical support for optimizing P.sinese intercropping cultivation system,and establishing scientifically intercropping model.[Method]The bacterial community diversity and structural differences in rizosphere soil when P.sinese was individually plant or intercropped with S.viciifolia were evaluated through Illumina Miseq high-throughput sequencing,Alpha diversity analysis,Beta diversity analysis,analysis about the combination of bacterial community,linear discriminant analysis(LDA),and correlation analysis.[Result]Intercropping P.sinese with S.viciifolia significantly increased available potassium in soil by 44.45%while decreasing available phosphorus,total phosphorus,total nitrogen and nitrate nitrogen contents by 35.88%,8.54%,16.99%and 51.91%,respectively,compared with P.sinese monoculture.The bacterial richness indices(Chao1 and ACE)showed significant increases of 1.80%and 1.97%,whereas no significant differences were observed in Shannon index,Simpson index or coverage.Distinct differences in microbial community structure of rhizosphere soil between monoculture and intercropping systems.Taxonomic analysis at both phylum and genus levels revealed that intercropping P.sinese with S.viciifolia significantly increased the relative abundances of Planctomycetes,Actinobacteria,and Gemmatimonadetes,while significantly decreasing Proteobacteria,Acidobacteria,Chloroflexi,and Verrucomicrobia compared to P.sinese monoculture.At the genus level,Streptomyces and AKYG587 showed significant enrichment,whereas Gemmata,RB41,and Candidatus_Udaeobacter were markedly reduced.Soil bacterial communities in monoculture systems were primarily influenced by organic matter,total potassium and moisture content,whereas in the intercropping system,the microbial community structure was mainly shaped by pH,total phosphorus,total nitrogen and ammonium nitrogen concentrations.[Conclusion]The intercropping system of P.sinese and S.viciifolia enhanced supply capacity of soil potassium and soil bacterial abundance,and improved bacterial community structure.At the phylum level,the relative abundances of Planctomycetes,Actinobacteria,and Gemmatimonadetes were significantly increased,while those of Proteobacteria,Acidobacteria,Chloroflexi and Verrucomicrobia were significantly decreased.At the genus level,Streptomyces and AKYG587 showed significant enrichment,whereas Gemmata,RB41,and Candidatus_Udaeobacter were markedly reduced.The bacterial community structure in the intercropping system was primarily governed by pH,total phosphorus,total nitrogen,and ammonium nitrogen concentrations.