草地贪夜蛾1龄虫取食胁迫对高粱幼苗叶片和根部转录组的影响OA

[Objective]The effects of 1st-instar Spodoptera frugiperda feeding on leaf and root transcriptomes of sorghum seedlings were explored to provide a theoretical basis for illustrating the molecular mechanism in response of sorghum seedlings to S.frugiperda feeding and breeding insect-resistant sorghum varieties.[Method]The leaf and root samples of Hongliangfeng 1(a sorghum variety)seedlings fed by 1st-instar S.frugiperda for 9 h(TR)were analyzed for transcriptome sequencing by taking normal Hongliangfeng 1 seedlings as CK.[Result]A total of 1 509 differentially expressed genes(DEGs)of 1 202 up-regulated and 307 down-regulated DEGs were identified in leaves,and 2 128 DEGs of 1 493 up-regulated and 635 down-regulated DEGs were identified in roots,which showed that DEGs were of tissue-specificity.The up-regulated genes in leaves were primarily involved in defense-related pathways of secondary metabolite synthesis,fatty acid metabolism,jasmonic acid biosynthesis,and so on,and the down-regulated genes in leaves were associated with DNA repair and reproduction.The up-regulated genes in roots were enriched in pathways of phenylpropanoid biosynthesis,cutin and waxiness synthesis,and phytohormone signaling transduction,and the down-regulated genes were related to growth and transport process.[Conclusion]The leaves of sorghum seedlings respond to S.frugiperda feeding stress by activating defense metabolic pathways and preferentially allocating defense resource,while the roots involves in defense by signal reception,synergistic response,and resource reallocation.