生姜组培苗后代群体的性状分析OA

[Objective]The variation mechanism of ginger tissue culture seedlings was investigated to effectively select the mutant seedlings for reducing the cost in subculture,transplanting and later management and lay the foundation for innovation of ginger germplasm resources.[Method]The differences between mutant and normal tissue culture plants were analyzed from the angles of phenotypic traits,cytology and molecular level.[Result]The mutant plants from M'3 generation showed dwarf,more branch number and lower rhizome weight,and no significant difference in fresh leaf weight compared with the normal plant.A total of 98 bands with a polymorphism ratio of 77.36%and polymorphism information content(PIC)of 0.646 5-0.890 0 were amplified by 12 pairs of SRAP primers.The genetic identity and genetic distance of four ginger populations(tissue culture seedling,breeder's seed,original seed and production seed)were 0.674 7-0.861 0 and 0.149 6-0.393 5 separately.The chromosome number of root tip cells in normal(M3)and mutant(M'3)plants both was 22(2n=2x=22).[Conclusion]There is a significant variation in phenotypic characteristics of plants from ginger production seed and no variation in chromosome number of plants from ginger production seed.At the molecular level,there is an extensive somatic cell variation in the offspring of ginger tissue culture seedlings.