金银花CBF/DREB1基因家族的鉴定与功能分析OA

[Objective]The structure,composition and characteristics of CBF/DREB1 gene family members of honeysuckle,and their expression patterns in different tissues and under abiotic stress were investigated to provide a theoretical basis for improving the stress resistance,yield and quality of honeysuckle.[Method]The Blast matching were conducted in the protein database of honeysuckle by using a bioinformatics method and taking the amino acid sequences of 6 CBF/DREB1 family members of Arabidopsis thaliana as templates,and the physicochemical properties and characteristics of LjCBFs were analyzed.The expression patterns of LjCBFs in different tissues were analyzed based on the transcriptome data in the NCBI database.The seedlings of Jinhua 3(a honeysuckle variety)grew under different abiotic stress respectively,and the flower buds were collected to analyze the expression patterns of LjCBFs.[Result]16 CBF/DREB1 gene family members identified from honeysuckle all were hydrophilic proteins,did not contain transmembrane domains and signal peptides,and contained 1-2 exons and 3-9 conserved motifs.Among them,10 were acidic proteins and 6 were basic proteins.The LjCBF1.1 protein had a relatively good stability only.The phylogenetic tree analysis showed that 61 LjCBFs were distributed in Group I,while LjCBF1.6 was located in Group II.The tissue-specific expression analysis showed that LjCBF1.1,LjCBF1.5,LjCBF1.10 and LjCBF1.12 were expressed in 9 tissues including leaves,stems,shoots,flowers and so on.The qRT-PCR detection analysis showed that LjCBF1.2 and LjCBF1.8 were significantly upregulated in response to 6 different abiotic stresses including high temperature,low temperature,salt,drought,osmosis and LiCl.[Conclusion]There are 16 CBF/DREB1 gene family members in the whole-genome of honeysuckle.Among them,LjCBF1.1,LjCBF1.5,LjCBF1.10 and LjCBF1.12 were expressed in 9 tissues at different developmental stages of honeysuckle,which indicates that they may play an important role in growth and development of honeysuckle.LjCBF1.2 and LjCBF1.8 show significant responses to six different abiotic stresses and are expressed in multiple tissues.It is speculated that they are involved in the response to abiotic stresses and growth and development regulation processes of honeysuckle.