雪豹粪便微生物组成分析与宿主健康的关联研究OA

In order to further understand the health status of snow leopards,one fecal sample was collected from a captive snow leopard(Panthera uncia)at Lasa Wildlife Park,and their intestinal microbial communities were analyzed in detail by 16S rRNA gene sequencing technology.A total of 125 958 high-quality sequences were obtained,and the average effective combination sequence of the sample was 109270.At the phylum level,the dominant intestinal microbiota of snow leopards was Firmicutes,followed by Bacteroidota.At the genus level,Peptoniphilus_methioninivorax was the dominant microbiota in the snow leopard.Among the alpha diversity indexes,the Chao1 index of snow leopard was 1 209.84,the ACE index was 1 327.63,the Shannon index was 4.57,and the Simpson index was 0.91,indicating that the diversity and uniformity of microbial communities were high.Predictive analysis of intestinal microbiota function in snow leopards showed that:these microorganisms were mainly involved in the metabolism of carbohydrates,amino acids,terpenoids,polyketides,cofactors and vitamins,which were essential for supporting the health of the host.Through in-depth analysis of the fecal microbial community of snow leopards,the study provides a strong theoretical support for assessing the health status of snow leopards and the ecosystem of their habitats.This not only helps to understand the physiological state of snow leopards in the natural environment,but also provides a scientific basis for the formulation of its protection measures.