西藏天仙子不同部位的红外光谱特性OA

To achieve the identification and quality control of different parts of Hyoscyamus niger L.from Xizang,this study obtained the raw infrared spectra of five parts:seeds,roots,stems,leaves,and fruit pods,using Fourier Transform Infrared Spectroscopy(FT-IR).The spectra were preprocessed,using OMNIC 9.0 software,and the relevant infrared spectral graphs were plotted,using OriginPro 2024 software.Cluster analysis of the second derivative infrared spectra(1 800～600 cm-1)was performed,using IBM SPSS Statistics 29.0.The results showed that there were significant differences between the original infrared spectra and the second derivative infrared spectra of the five different parts of Hyoscyamus niger L.The second derivative infrared spectra showed greatest similarity between seeds and leaves(coefficient:0.3 456),whereas cluster analysis grouped the seeds into a distinct cluster.The absorption peaks of seeds at 1 752 and 1 741 cm-1 were sharp and strong,allowing for their differentiation from the other four parts.The absence of an obvious absorption peak at 1 632 cm-1 in the roots helped distinguish them from the seeds.For leaves,the absorption peaks at 1 387 and 1 170 cm-1 were not obvious,allowing for differentiation of seeds from leaves.Additionally,the lack of significant absorption peaks for seeds at 785 and 697 cm-1 enabled their differentiation from stems and fruit pods.Therefore,rapid identification of the five different parts of Hyoscyamus niger L.can be achieved through infrared spectroscopy.The findings of this study provide insights into the chemical information of different parts of Hyoscyamus niger L.,with a fast,simple,and accurate method for identification and a basis for the quality control and application development of Hyoscyamus niger L.resources.