电针"内关"和"足三里"对MCAO大鼠皮层神经再生相关蛋白表达的影响OA

Objective:To explore the effects of electroacupuncture at"Neiguan"and"Zu sanli"on the growth asso-ciated protein-43(GAP-43)and the Nogo-A/NgR signaling pathway(including neurotrophic inhibitory proteins and their receptors)in axonal regeneration within the infarcted cerebral cortex of rats with middle cerebral artery occlusion(MCAO).Methods:Healthy male SPF rats were randomly divided into a sham operation group,a model group,and an electroacupuncture group.The model group and the electroacupuncture group were used to establish the MCAO model in rats using the Zea Longa modified suture method,while in the sham operation group,only the artery was exposed without occlusion.In the electroacupuncture group,rats received electroacupuncture treatment at bilateral Neiguan and Zu sanli acupoints for 20 minutes once daily,starting 24 hours after model establishment.The sham operation group and the model group received no intervention.Brain tissue was collected from each group for analysis at 3 days,7 days,and 14 days after treatment.After fresh tissue collection,protein expression of GAP-43,Nogo-A,and NgR was detected by Western blot,and the mRNA expression of Nogo-A and NgR were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results:Compared with the model group,the electroacupuncture group showed increased GAP-43 expression at days 3,7,and 14 after treatment(P<0.05),while the protein expression of Nogo-A and the mRNA expression of Nogo-A and NgR were decreased(P<0.05),the protein expression of NgR is dencreased at days 14.Conclusion:Electroacupuncture at"Neiguan"and"Zu sanli"can inhibit the expression of Nogo-A/NgR signaling pathway-related proteins and their mRNA in the infarcted cerebral cortex,and upregulate the expression of GAP-43 in the infarcted cerebral cortex,thereby reducing nerve damage.