印度獐牙菜化学成分研究OA

Swertia chirayita,referred to as"Jati"in Tibetan medicine,is traditionally employed for the treatment of hepatic and biliary disorders and has demonstrated significant efficacy in managing diabetes.To explore the chemical constituents and biological activities of the medicinal plant S.chirayita,various chromatographic techniques were applied to isolate and purify the ethyl acetate fraction of the plant extract.The structures of the isolated compounds were identified using modern spectroscopic methods(NMR,MS).Furthermore,the glucose uptake and antioxidant activities of the isolated some compounds were evaluated.(1)Twenty compounds were isolated from the ethyl acetate extract,including twelve xanthone derivatives,two iridoids,and two flavonoids.Twenty compounds were identified as bellidifolin(1),1,3,5,8-tetrahydroxyxanthenone(2),1,8-dihydroxy-3,7-dimethoxyxanthone(3),1,8-dihydroxy-3,5-dimethoxyxanthone(4),1-hydroxy-3,5,8-trimethoxyxanthone(5),1,7,8-trihydroxy-3-methoxyxanthone(6),1-hydroxy-3,7,8-trimethoxyxanthone(7),1-hydroxy-3,5-dimethoxyxanthone(8),1,3,8-trihydroxy-7-methoxyxanthone(9),norswertianin(10),1,3,7-trihydroxyxanthone(11),1,3,5-trihydroxyxanthone(12),amaroswerin(13),amarogentin(14),luteolin(15),quercetin(16),(+)-syringaresinol(17),gentianine(18),swerimilegenin C(19),and dehydroxyl-swerimilegenin H(20).Among them,compounds 4,6,7,9,12,15,17,19,20 were isolated for the first time from S.chirayita.Distinct from congeneric species such as S.mileensi(characterized by monoterpene glycosides and polyhydroxy xanthones),S.chirayita exhibits a unique phytochemical profile dominated by polymethoxylated xanthones and iridoid glycosides.This chemotaxonomic divergence likely reflects ecological adaptations to its high-altitude habitat and underpins its traditional therapeutic efficacy in treating metabolic disorders.(2)The glucose uptake assay showed that compounds 2,4,6,and 10-14 had good glucose uptake activities;the intracellular ROS measurement results indicated that compounds 1 and 7 had strong inhibitory effects,while compounds 10 and 14 had good promoting effects.This study enriches the chemical profile of S.chirayita and further clarifies its constituents that affect glucose uptake and exhibit antioxidant activities,providing a chemical foundation for further pharmacological research.