用于血管紧张素-Ⅱ和L-NAME诱导的高血压小鼠模型脑血管动力学超分辨率成像的多模态超快超声显微成像(MUSM)OA

Hypertension is a major cause of cerebral hemorrhage.Although they are widely used in preclinical studies on cerebral hemorrhage,traditional in vivo cerebrovascular imaging techniques,such as positron emission tomography and magnetic resonance imaging,often fall short in dynamically visualizing cerebral microcirculation blood flow in rodent models.This study leveraged the high spatiotemporal resolution of multimodal ultrafast sonography microscopy(MUSM)to assess cerebrovascular hemodynamics in vivo within hypertensive mice induced by Angiotensin Ⅱ(Ang Ⅱ)and Nx-nitro-L-arginine methyl ester(L-NAME).Cerebrovascular hemodynamics were quantified using variations in cerebral vascular density,diameter,velocity,tortuosity,cerebral flow pulsatility,and instant flow direction.Our findings revealed a decrease in cerebral vascular density and perfusion index after blood pressure increased,particularly in the cortex and basal ganglia regions.This study not only provides a comprehensive view of cerebral dynamics in hypertension but also introduces MUSM as a novel tool for in vivo cerebrovascular hemodynamic analysis in preclinical animal research.