EPPK1蛋白在子宫内膜癌中的表达及其分子病理学意义OA

Objective To investigate expression profile of epiplakin 1(EPPK1)in endometrial carcinoma and its potential clinical significance.Methods Based on The Cancer Genome Atlas(TCGA)database,using the R Programming Language software,analysis of mRNA expression levels of EPPK1 in endometrial carcinoma tissues was conducted to analyze correlations between EPPK1 expression and clinical-pathological parameters such as patient age and tumor grade.Kaplan-Meier survival analysis was performed to explore associations of EPPK1 expression with overall survival(OS)and progression-free survival(PFS).To further validate EPPK1 expression status in endometrial carcinoma tissues,immunohistochemistry(IHC)for tissue microarrays containing endometrial carcinoma tissues and adjacent non-cancerous tissues was conducted and scored based on proportion of the positive cells and staining intensity.Statistical analysis was performed using Mann-Whitney U test.Additionally,UALCAN and cBioPortal databases were used to characterize EPPK1 expression patterns and gene alterations,and TIMER2.0 database was further employed to assess its expression trends across multiple cancer types.Results EPPK1 was significantly upregulated in endometrial carcinoma tissues and closely correlated with patient age and tumor grade,and the patients with high EPPK1 expression exhibited poor OS(HR=1.91,95%CI:1.26-2.09,P=0.020)and PFS(HR=1.51,95%CI:1.07-2.14,P=0.02).IHC further confirmed upregulation of EPPK1 expression level in endometrial carcinoma tissues.Moreover,EPPK1 expression was potentially associated with expression of key proteins in multiple signaling pathways and exhibited a high expression trend across many different cancer types.Conclusion EPPK1 is highly expressed in endometrial carcinoma tissues and may participate in tumorigenesis and progression.It holds promise as a potential diagnostic and prognostic biomarker.