铁铈双金属有机框架的合成及其酶催化抗菌性能研究OA

Objective To provide experimental evidence and theoretical basis for their applications in the antibacterial field,iron-cerium bimetallic organic frameworks(FeCeMOF)were prepared.It's enzyme-catalytic antibacterial properties were systematically investigated.Methods FeCe-MOF was prepared by a one-pot method.The material was characterized by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy and other techniques to verify the distribution of Fe,Ce and related elements in the organic ligand,as well as the structural characteristics of the material.The peroxidase-like(POD),catalase-like(CAT)and glutathione peroxidase-like(GPx)activities,together with POD-like enzymatic kinetics,were systematically investigated to evaluate the nanozyme catalytic activity of FeCe-MOF.Using Methicillin-resistant Staphylococcus aureus(MRSA)and Escherichia coli(E.coli)as test strains,in vitro antibacterial experiments were carried out via the plate colony counting method and live/dead fluorescence staining,to study the antibacterial performance and effect of FeCe-MOF.Results Elemental mapping analysis confirmed the successful synthesis of FeCe-MOF,with Fe,Ce,and elements related to the organic ligands showing uniform distribution within the material.Nanozyme catalytic activity tests demonstrated that FeCe-MOF possesses the ability to generate reactive oxygen species(ROS)and can promote ROS accumulation by consuming intracellular glutathione(GSH)in bacteria.Elevated intracellular ROS levels induce a series of oxidative stress responses in bacteria,thereby exerting antibacterial effects.In vitro antibacterial experiments revealed that FeCe-MOF exhibits excellent antibacterial activity against MRSA and E.coli.Conclusion FeCe-MOF exerts bactericidal effects by generating reactive oxygen species(ROS)through its nanozyme activity.Thus,it holds great promise as a novel antibacterial material.