白术内酯Ⅰ对白塞病小鼠脾脏Th17/Treg平衡的影响OA

Objective:To study the effects of atractylenolide I on spleen Th17/Treg balance in Behçet's disease(BD)mice.Methods:Sixty mice were randomly assigned to three groups:a blank control group,a model group and an experiment group with 20 in each.The models were then established following the method described in the literature.Mice in the blank control group and the model group were administered an equal volume of 0.9%saline,while the experiment group received atractylenolide I.Flow cytometry was adopted to detect the number of Th17 and Treg cells in the spleen;ELISA method was employed to measure the levels of Th17 cytokines IL-6,IL-17,and the expressions of Treg cytokines IL-4 and IL-10 in the spleen of the mice in all the groups;Western blotting method was applied to determine the levels of critical Th17 regulator RORγt and critical Treg regulator Foxp3 in the spleen of the mice.Results:Compared with the blank control group,the increased expressions of Th17,IL-6,IL-17 and RORγt,and Th17/Treg ratio was observed(P<0.05);while the downregulation of IL-10,Treg and Foxp3 was detected in the model group(P<0.05).Compared with the model group,the experiment group exhibited the decreased expressions of Th17,IL-6,IL-17 and RORγt,and Th17/Treg ratio(P<0.05);and the upregulation of IL-10,Treg and Foxp3(P<0.05).Conclusion:Atractylenolide I could improve immune function in tropomyosin-induced BD mice possibly through regulating Th17/Treg imbalance.