mt-Pro 5'tiRNA在C2C12细胞增殖中的作用及靶基因预测OA

tiRNAs(stress-inducible RNAs derived from tRNA)play a pivotal role in the pathogenesis and progression of various diseases.It is known that mitochondrial proline tRNA-derived mt-Pro 5'tiRNA exhibits high expression levels in skeletal muscle,yet its potential involvement in the repair and regeneration of skeletal muscle injury in mice remains unclear.C2C12 cells were used as experimental material.The expression changes of mt-Pro 5'tiRNA during cell proliferation and differentiation were detected using RT-qPCR,while the effects of mt-Pro 5'tiRNA on cell viability were assessed by the CCK-8 assay.Additionally,candidate target genes were predicted.The results demonstrated that the expression of mt-Pro 5'tiRNA was reduced during C2C12 cell proliferation but increased during C2C12 cell differentiation.Overexpression of mt-Pro 5'tiRNA decreased cell viability.Bioinformatics analysis identified 2 273 candidate target genes,and KEGG pathway analysis revealed significant enrichment of these genes in the Hippo signaling pathway,Wnt signaling pathway,and MAPK signaling pathway.The findings lay the foundation for further mechanistic studies on the role of mt-Pro 5'tiRNA in muscle injury repair.