不同核酸提取方法对肉松和肉丸中非洲猪瘟病毒微滴式数字PCR检测结果的影响OA

In order to develop a droplet digital PCR method for detection of African swine fever virus in deep processed meat products such as meat floss and meatballs,the aim was to study the effect of different extraction methods on the extraction efficiency of African swine fever virus DNA.By comparing the effects of different nucleic acid extraction methods on the droplet digital PCR detection re-sults of the virus.The methods evaluated included the phenol-chloroform DNA extraction method,the rapid extraction method using the TaKaRa MiniBEST Viral RNA/DNA Extraction Kit,the rapid extraction method using the QIAGEN DNeasy○R mericon Food Kit,and the rapid extraction method using the TIANGEN Namp Genomic DNA Kit.TaKaRa MiniBEST Viral RNA/DNA Extraction Kit and QIAGEN DNeasy○R mericon Food Kit have a relatively good effect.Based on the complexity of the extraction program and the influ-ence of the heating and cracking process,it was ultimately confirmed that the TaKaRa MiniBEST Viral RNA/DNA Extraction Kit rapid extraction method is the most suitable solution.