1株鹅源食窦魏斯氏菌的筛选及其益生特性研究OA

This study aimed to screen lactic acid bacteria with strong acid-producing capacity and favorable pro-biotic properties from the feces of healthy geese,laying a foundation for their future application in intestinal health management of geese.Initially,lactic acid bacteria were isolated and purified from healthy goose feces using MRS solid medium supplemented with calcium carbonate(CaCO3).Strains exhibiting strong acid-produ-cing capacity were selected and subjected to molecular biological identification to determine their species.Sub-sequently,their growth characteristics,tolerance to acid and bile salts,antibiotic susceptibility,antimicrobial activity,antioxidant activity,and safety were further evaluated.Finally,the effects of the selected strain on the growth performance and diarrhea rate of goslings were assessed.The results showed that a high acid-producing lactic acid bacteria strain F11 was successfully isolated from healthy goose feces.Based on 16S rDNA sequence analysis,it was identified as Weissella cibaria.In terms of growth characteristics,strain F11 was in the loga-rithmic growth phase during 2 to 8 h of cultivation.Regarding tolerance,it was able to grow under conditions of pH 3.0 and 0.3%bile salt concentration,demonstrating good gastrointestinal environmental tolerance.The strain exhibited inhibitory activity against Staphylococcus aureus,Salmonella typhimurium,Pseudomonas aeruginosa,and Escherichia coli,and its fermentation supernatant also showed certain antioxidant activity.An-tibiotic susceptibility testing indicated that strain F11 was sensitive to florfenicol,doxycycline,clindamycin,chloramphenicol,cephalexin,gentamicin,ceftriaxone,tetracycline and minocycline,and not sensitive to van-comycin,norfloxacin,piperacillin,kanamycin,oxacillin,streptomycin and cefazolin.Furthermore,the strain showed no hemolytic activity.The animal trial results showed that strain F11 could improve the growth per-formance and reduce the diarrhea rate of goslings.In summary,Weissella cibaria F11,isolated from healthy goose feces in this study,demonstrates favorable probiotic properties.The strain possesses stable acid-produ-cing capability,strong tolerance to gastrointestinal conditions,inhibitory effects against common pathogenic bacteria,and an antibiotic resistance profile that meets probiotic safety standards.Furthermore,it effectively enhances the growth performance and lowers the diarrhea rate in goslings.Therefore,Weissella cibaria F11 ex-hibits promising potential for development as an intestinal probiotic for geese.[Chinese Journal of Animal Nu-trition,2026,38(4):3066-3076]