渝东黑山羊和板角山羊背最长肌营养组分及肌内脂肪转录组分析OA

This experiment aimed to compare the differences in nutritional components in longissimus dorsi muscle between Yudong black goats and Banjiao goats,and to screen for genes related to intramuscular fat(IMF)deposition in goats through transcriptome sequencing(RNA-Seq).Six male Yudong black goats and six male Banjiao goats with similar body weight of(24.00±2.12)kg at 10 months of age under the same feed-ing and management conditions were selected.After 60 days of feeding(10 days for the pre-test period and 50 days for the formal test period),they were slaughtered.The longissimus dorsi muscle was collected to deter-mine the nutritional components and perform RNA-Seq.The results showed as follows:1)the IMF content in longissimus dorsi muscle of Yudong black goats was significantly higher than that of Banjiao goats(P＜0.05),while the yellowness(b*)value and shear force were significantly lower than those of Banjiao goats(P＜0.05).2)There was no significant difference in the amino acid composition and contents in longissimus dorsi muscle between the two breeds of goats(P＞0.05).The contents of vitamin E,zinc and total polyunsaturated fatty acids in longissimus dorsi muscle of Yudong black goats were significantly higher than those of Banjiao goats(P＜0.05),while the contents of potassium,magnesium and total saturated fatty acids were significantly lower than those of Banjiao goats(P＜0.05).3)RNA-Seq analysis revealed that compared with Banjiao goats,the longissimus dorsi muscle of Yudong black goats had a total of 1 208 candidate differentially ex-pressed genes(827 up-regulated and 381 down-regulated),which were significantly enriched in fat deposition-related pathways such as the peroxisome proliferator-activated receptor signaling pathway and fatty acid biosyn-thesis.Additionally,four candidate genes were further screened out:fatty acid desaturase 1(FADS1),solute carrier family 27 member 1(SLC27A1),peroxisome proliferator-activated receptor δ(PPARD),and perilip-in 2(PLIN2).4)The verification results of real-time fluorescence quantitative PCR(RT-qPCR)were consist-ent with the results of RNA-Seq.The relative expression levels of FADS1 and SLC27A1 in longissimus dorsi muscle of Yudong black goats were significantly lower than those of Banjiao goats(P＜0.05),and the relative expression levels of PPARD and PLIN2 in longissimus dorsi muscle were significantly higher than those of Banjiao goats(P＜0.05).In conclusion,compared with Banjiao goats,Yudong black goats have richer muscle nutrients and better meat quality.The four fat deposition-related genes screened by RNA-Seq provide a refer-ence for the regulatory mechanism of IMF deposition in meat-type goats.[Chinese Journal of Animal Nutri-tion,2026,38(4):2979-2993]