大豆S40家族生物信息学分析及盐碱干旱胁迫下表达模式分析OA
Bioinformatics Analysis of Soybean S40 Family and Expression Analysis Under Abiotic Stress
S40 基因家族属于 DUF584 家族,其编码蛋白含有 DUF584 结构域.为解析大豆 S40 基因在盐碱、干旱等非生物胁迫应答中的功能,对大豆基因组中含 DUF584 结构域的基因进行全基因组鉴定,并系统开展家族进化树构建、结构域组成、基因结构、启动子顺式作用元件、共线性及组织特异性表达分析,检测其在盐碱、干旱及渗透胁迫下的表达模式.结果显示:在大豆基因组中共鉴定出30 个S40 家族成员,分布于13 条染色体上.理化性质分析显示,大豆S40基因家族编码蛋白的氨基酸长度为49～266 aa,分子量为5.43～28.96 kDa.系统发育分析将大豆、拟南芥和水稻的S40 基因划分为5 个亚家族,其中 G1 与 G5 亚家族成员数量最多.结构域分析表明,所有 S40 成员均只含有 DUF584保守结构域,但结构域在序列中的位置存在差异,提示该家族成员可能存在功能分化.基因结构分析显示,不同成员的外显子-内含子数量与排布存在明显差异,进一步说明其功能具有多样性.启动子顺式作用元件分析发现,S40 家族成员含有光响应、生长发育、激素调控及逆境胁迫相关元件,推测该家族参与大豆非生物胁迫应答过程.共线性分析显示,同一亚家族内成员间亲缘关系较近、保守性较高.逆境表达分析表明,G5 亚家族基因 GmS40-3 在盐碱、干旱及渗透胁迫下的表达水平变化最为显著.研究结果为大豆 S40 基因家族的功能解析提供了理论基础,也为进一步阐明大豆逆境适应机制和改良大豆耐逆性提供了参考.
The S40 gene family belongs to the DUF584 family,and its encoded proteins contain the DUF584 domain.To elucidate the function of the soybean S40 gene in responses to abiotic stresses such as salt-alkali and drought,this study conducted a genome-wide identification of genes containing the DUF584 domain in the soybean genome,and systematically carried out analyses including the construction of a family evolutionary tree,domain composition,gene structure,cis-acting elements in the promoter,synteny analysis,and tissue-specific expression.Finally,the expression patterns of these genes under salt-alkali,drought,and osmotic stress were detected.The results showed that a total of thirty S40 family members were identified in the soybean genome,distributed on 13 chromosomes.Physical and chemical property analysis indicated that the amino acid lengths of the encoded proteins of the soybean S40 gene family ranged from 49 to 266 aa,and the molecular weights were between 5.43 and 28.96 kDa.Phylogenetic analysis classified the S40 genes of soybean,Arabidopsis,and rice into five subfamilies,among which the G1 and G5 subfamilies had the largest number of members.Domain analysis revealed that all S40 members contained only the conserved DUF584 domain,but the positions of the domain in the sequences were different,suggesting possible functional diversification among the family members.Gene structure analysis showed that the number and arrangement of exons and introns in different members were significantly different,further supporting their functional diversity.Promoter cis-acting element analysis found that S40 family members contained light response,growth and development,hormone regulation,and stress-related elements,suggesting that this family is involved in the response of soybean to abiotic stresses.Synteny analysis indicated that members within the same subfamily had closer genetic relationships and higher conservation.Expression pattern analysis under adverse conditions showed that the expression level of the G5 subfamily gene GmS40-3 changed most significantly under salt-alkali,drought,and osmotic stress.This study provides a theoretical basis for the functional analysis of the soybean S40 gene family and also offers reference for further clarifying the mechanism of soybean adaptation to adverse conditions and improving its stress tolerance.
张业奇;刘洋;张滨烁;韩敬佩;武小霞;赵莹
东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030东北农业大学 农学院/教育部大豆生物学重点实验室,黑龙江 哈尔滨 150030
大豆S40生物信息学分析非生物胁迫表达分析
soybeanS40bioinformatics analysisabiotic stressexpression analysis
《大豆科学》 2026 (2)
13-24,12
国家重点研发计划(2023YFD2300101-02)黑龙江省自然科学基金(YQ2022C010,TD2022C003).
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