溃疡性结肠炎患者肠道菌群紊乱与病理分型的相关性OA

Objective:To investigate the correlation between gut microbiota dysbiosis and histological activity grading in patients with ulcerative colitis(UC).Methods:A total of 120 patients UC were sellected as the research subjects.According to the Nancy histological index,patients were classified into a mild activity group(score 2,n=58)and a severe activity group(score 4,n=62).The scores of crypt abscesses,inflammatory cell infiltration,glandular destruction,and composite histological were compared between the two groups.Previously obtained 16S rRNA sequencing data were used to quantify the relative abundance of key bacterial taxa(Firmicutes,Proteobacteria,Faecalibacterium,Bifidobacterium,and Escherichia-Shigella).Spearman correlation analysis was performed to assess the associations between major taxa and inflammatory indicators,including C-reactive protein(CRP),rythrocyte sedimentation rate(ESR),Mayo clinical activity index(Mayo score),and composite histological score.Results:Compared with the mild activity group,patients in the severe activity group exhibited lower hemoglobin(Hb)and albumin(Alb)levels(P<0.05),and higher white blood cell count(WBC),platelet count(PLT),CRP,erythrocyte sedimentation rate(ESR),and fecal occult blood test(FOBT)positivity(P<0.05).Histologically,the severe activity group showed higher scores for crypt abscesses,inflammatory cell infiltration,glandular destruction,and composite histological severity(P<0.001).Microbiota analysis revealed that the relative abundance of Firmicutes,Faecalibacterium,and Bifidobacterium was lower in the severe activity group,whereas Proteobacteria and Escherichia-Shigella showed higher relative abundance(P<0.05).Spearman correlation analysis demonstrated that Escherichia-Shigella was positively correlated with CRP,ESR,Mayo score,and composite histological score(P<0.01).In contrast,Faecalibacterium showed moderate negative correlations with these indicators(P<0.05),while Bifidobacterium exhibited mild-to-moderate negative correlations with CRP,Mayo score,and composite histological score(P<0.01).Conclusion:Distinct gut microbiota profiles are observed between different histological activity grades of UC.Patients with severe histological activity exhibit higher relative abundance of pathogenic taxa(Escherichia-Shigella and Proteobacteria)and lower relative abundance of beneficial taxa(Faecalibacterium and Bifidobacterium).The consistent associations between major bacterial taxa and inflammatory as well as histological indicators suggest that gut microbiota features may serve as potential microbial markers reflecting the severity of mucosal inflammation in UC,providing valuable reference for disease assessment.