基质栽培对兰州百合茎生小鳞茎发育的激素调控机制研究OA
Hormonal regulation of stem-derived bulblet development in Lilium davidii var.unicolor cultivated in a peat-perlite substrate
为探究不同栽培基质对兰州百合鳞茎增殖的影响及其激素调控机制,以兰州百合为试验材料,设置纯沙壤土(CK)和泥炭∶珍珠岩=1∶1(T1)两个处理,测定兰州百合生长指标并采用超高效液相色谱—串联质谱法(UPLC-MS/MS)检测茎生小鳞茎发育处的内源激素含量.结果表明:T1处理显著促进兰州百合生长,开花期(6月15日)株高(41.47 cm)较CK(36.53 cm)增加13.5％,茎粗(7.97 mm)较CK(6.50 mm)增加22.6％;T1处理茎生小鳞茎数量明显多于CK.激素含量分析显示,T1处理下茉莉酸甲酯(4.76 ng·g-1)、N6-异戊烯腺嘌呤(0.63 ng·g-1)和赤霉素A12醛(3.04 ng·g-1)含量较 CK(1.90、0.30、0.90 ng·g-1)显著上调(P＜0.05),而色胺(1.69 ng·g-1)、水杨酸-2-O-β-葡萄糖苷(229.71 ng·g-1)等含量较CK(3.54、530.19 ng·g-1)显著下调(P＜0.05).代谢通路分析表明,差异激素主要富集于植物激素信号转导、二萜类物质生物合成等20条通路,其中赤霉素A12醛、N6-异戊烯腺嘌呤可能为调控鳞茎发育的关键节点.研究证实,泥炭∶珍珠岩=1∶1的基质可能通过调节细胞分裂素、赤霉素和茉莉酸的合成与转运,显著促进了兰州百合茎生小鳞茎增殖(P＜0.05),为其种球规模化生产提供了理论依据.
The aim of this work was to explore the effects of the cultivation substrate on bulb proliferation of Lanzhou lily(Lilium davidii var.unicolor)and to clarify its hormonal regulation mechanisms.Lanzhou lily plants were grown in two substrates:pure sandy loam soil(CK)and a mixture of peat and perlite at a 1∶1 ratio(T1).During the experiment,growth indices of Lanzhou lily were measured,and the endogenous hormone contents in developing bulblets were detected by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The results show that the T1 treatment significantly promoted the growth of Lanzhou lily.At the flowering stage(June 15),compared with the plants in CK,those in the T1 treatment showed 13.5％greater plant height(41.47 cm vs.36.53 cm)and 22.6％greater stem diameter(7.97 mm vs.6.50 mm).The number of bulblets was significantly higher in the T1 treatment than in CK.The contents of methyl jasmonate(4.76 ng·g-1),N6-isopentenyladenine(0.63 ng·g-1),and gibberellin(GA)A12 aldehyde(3.04 ng·g-1)were significantly higher in plants in the T1 treatment than in those in the CK(1.90,0.30,and 0.90 ng·g-1,respectively)(P＜0.05).The contents of tryptamine(1.69 ng·g-1)and salicylic acid-2-O-β-glucoside(229.71 ng·g-1)were significantly lower in plants in the T1 treatment than in those in the CK(3.54 and 530.19 ng·g-1,respectively)(P＜0.05).Metabolic pathway analysis indicated that the differentially accumulated hormones were mainly enriched in 20 pathways including plant hormone signal transduction and diterpenoid biosynthesis.Among the metabolites of these pathways,GA A12 aldehyde and N6-isopentenyladenine were identified as potential key regulatory nodes in bulb development.The results of this study confirmed that the peat∶perlite=1∶1 substrate significantly promoted the proliferation of Lanzhou lily caudex bulblets(P＜0.05)by regulating the synthesis and transport of cytokinin,gibberellin,and jasmonic acid.These findings provide a theoretical basis for the large-scale production of Lanzhou lily bulblets.
马祥臣;勉鹏飞;张萍;李不染;靳磊
宁夏大学林业与草业学院,宁夏银川 750021宁夏科育种苗有限公司,宁夏银川 750001宁夏大学林业与草业学院,宁夏银川 750021宁夏大学林业与草业学院,宁夏银川 750021宁夏大学林业与草业学院,宁夏银川 750021
兰州百合栽培基质茎生小鳞茎内源激素
Lilium davidii var.unicolorcultivation substratescauline bulbletsendogenous hormones
《草业学报》 2026 (4)
135-146,12
国家自然科学基金(32260727)和宁夏回族自治区重点研发计划项目(2021BBF02025)资助.
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