敲低MALAT1对人鼻咽癌HONE-1细胞增殖、迁移和侵袭的影响OA

Objective To investigate the effect of MALAT1 knockdown on the proliferation and migration and invasion in HONE-1 of nasopharyngeal carcinoma cells.Methods The nasopharyngeal carcinoma HONE-1 cells were divided into two groups,which were transfected with control plasmid(shcon group)and shMALAT1 plasmid(shMALAT1 group)respectively.The transfection rates of the two groups were detected by fluorescence microscope.And MALAT1 gene knockdown level was de-tected by real-time fluorescence quantitative PCR(qRT-RCR).The proliferation abilities were detected by CCK8 method;the migration and invasion ability of the two groups were detected by transwell assay;and the expression levels of vimentin and E-cadherin mRNA were detected by qRT-PCR;and the expression levels of vimentin and E-cadherin protein were detected by West-ern blot.Results Compared with the control group,the expressions level of MALAT1 was significantly decreased after transfection shMALAT1 plasmid.Compared with the control group,the cell proliferation ability(t=5.520,P＜0.000 1)and migration a-bility(t=9.285,P＜0.000 1;t=9.577,P＜0.000 1)and invasion ability(t=8.481,P＜0.000 1)in shMALAT1 group was significantly decreased,the expressions level of vimentin mRNA and protein in the shMALAT1 group were significantly de-creased.the expression levels of E-cadherin mRNA and protein in the shMALAT1 group were significantly increased.Conclusion Knocking down MALAT1 gene can inhibit proliferation,migration,invasion and EMT of HONE-1 cells in human nasopharyn-geal cancer.