大球盖菇耐高温菌株驯化及优良菌株筛选OA

Taking six Stropharia rugosoannulata strains(DQ1,DQ8,LS1,Golden Stropharia,CD1,CD2)as test materials,a progressive gradient high-temperature acclimation method(25℃(control)→28 ℃→ 31 ℃→ 34℃)was employed to study mycelial growth rate,mycelial vigor,mycelial dry mass,post-high-temperature stress recovery capacity,and high-temperature tolerance characteristics of each strain,in order to overcome temperature limitations on Stropharia rugosoannulata mycelial growth,screen superior strains suitable for high-temperature environments(28-34℃),and provide reference for cultivating Stropharia rugosoannulata in high-temperature regions and establishing year-round production systems.The results showed that as acclimation temperature increased,mycelial growth of all six strains was inhibited,but the degree of inhibition varied significantly(P＜0.05).During the severe high-temperature acclimation stage at 34℃,strains DQ1 and DQ8 performed the best,with mycelial growth rates reaching 0.43 cm·d-1 and 0.41 cm·d-1,respectively,significantly higher than other strains;mycelial vigor remained at level 4(dense and brown-free),with mycelial dry masses of 0.82 g·(100mL)-1 and 0.79 g·(100mL)-1;after 34℃ stress and transfer to 25℃cultivation for 24 hours,both strains achieved 100％mycelial recovery.Strains LS1 and Golden stropharia exhibited severe mycelial browning and growth stagnation above 31℃,while strains CD1 and CD2 showed moderate high-temperature tolerance.In conclusion,strains DQ1 and DQ8 demonstrated outstanding high-temperature resistance and could be selected as optimal strains for Stropharia rugosoannulata mycelial cultivation in high-temperature environments.