枣疯病对酸枣中类黄酮生物合成途径的差异基因表达分析OA

Taking jujube witches' disease-resistant healthy leaves(KB)and disease-susceptible leaves(ZFB)as experimental materials,the flavonoid content of KB and ZFB was determined by NaNO2-Al(NO3)3-NaOH colorimetric method.Transcriptome sequencing technology and qRT-PCR technology were used to screen for metabolic pathways and differentially expressed genes related to resistance to jujube disease,and quantitative verification was performed.The effects of jujube witches'-broom phytoplasma on the flavonoid biosynthesis pathway of sour jujube were studied,in order to provide reference for the breeding of sour jujube varieties.The results showed that a total of 4 682 differentially expressed genes(DEGs)were collected between KB and ZFB,of which 1 540 genes were upregulated and 3 142 genes were downregulated.In GO enrichment analysis,a total of 4 066 differentially expressed genes were annotated,including flavonoid biosynthesis and flavonoid metabolism processes.In the KEGG enrichment pathway,it was also significantly enriched in pathways such as flavonoid biosynthesis.Based on the annotations of 4 682 DEGs in the transcriptome database,25 key genes related to flavonoid biosynthesis were identified,including HCT and CCoAOMT genes,which were significantly upregulated in the KB vs ZFB group and could promote the accumulation of ferulic acid coenzyme A.There were 226 transcription factor family genes,mainly concentrated in ERF,MYB,bHLH families known to regulate flavonoid metabolism.