基于基因组和代谢组学的冬虫夏草菌虫草素生物合成代谢途径研究OA

[Objective]To investigate the biosynthetic pathway of cordycepin in Ophiocordyceps sinensis,and provide a theoretical basis for in-depth research and biotechnological application[Method]High-throughput sequencing technology was used to sequence and assemble the whole genome of O.sinensis.Potential key enzyme genes involved in cordycepin biosynthesis were predicted and identified through comparative ge-nomic analysis.The metabolic profile of O.sinensis fruiting bodies was analyzed by liquid chromatography-mass spectrometry(LC-MS),identif-ying differential metabolites associated with cordycepin synthesis.Furthermore,the possible biosynthetic pathway of cordycepin was inferred by integrating key gene functions,dynamic changes of differential metabolites,and KEGG pathway enrichment analysis.[Result]Chromosome-level genome assembly and preliminary analysis revealed that the O.sinensis genome size is 111.4 Mbp with a GC content of 45.43%and an N50 of 17.66 Mbp.A total of 9 519 protein-coding genes were identified,with an average length of 1 481 bp.Enzymes such as adenylate ki-nase,adenosine kinase,and ribonucleic acid reductase and 5'-nucleotidase were predicted to be involved in cordycepin biosynthesis.[Con-clusion]Through genomic and metabolomic integration analysis,the biosynthetic pathway network of cordycepin in O.sinensis was initially constructed at the systems biology level,and multiple key candidate genes and metabolic nodes were identified,laying a theoretical foundation for further verification of functional genes and the production of cordycepin using synthetic biology strategies.