小麦DUF4408基因家族的全基因组鉴定及表达分析OA

The DUF4408 gene family in wheat was identified through bioinformatics approaches with whole-genome data from the Ensemble Plants database.Analyses were conducted,including subcellular localization,phylogenetic relationships,gene structure,protein domains,promoter cis-elements,post-transcriptional regulation,syntenic analysis,and expression patterns.A total of 24 TaDUF4408 gene family mem-bers were identified.Phylogenetic analysis classified them into two distinct subgroups,and a variety of cis-acting regulatory elements associated with environmental stress responses and hormone signaling pathways were detected in the promoter regions.It was hypothesized that the wheat DUF4408 gene family may be involved in physiological processes such as light regulation,abscisic acid synthesis,and jasmonic acid methyl ester synthesis.miRNA target prediction revealed 13 miRNAs possibly targeting 15 TaDUF4408 genes.Syntenic analysis indicated that DUF4408 genes are relatively conserved through species evolution.Expression pattern analysis demonstrated significant differences in expres-sion levels among family members across different tissues,with distinct expression characteristics under drought and high-temperature stresses.