贝莱斯芽胞杆菌BS19增强烟草青枯病抗性的转录组分析OA

[Objective]This study aimed to elucidate the transcriptional regulatory mechanisms by which Ba-cillus velezensis BS19 induces tobacco resistance against bacterial wilt.[Method]Based on the Illumina high-throughput sequencing platform,transcriptomic differences in tobacco roots between the BS19-treated group and the control group were comparatively analyzed.[Result]BS19 treatment significantly induced 2 984 differen-tially expressed genes(DEGs),including 1 939 upregulated and 1 045 downregulated genes.GO enrichment analysis revealed that:DEGs in biological processes were primarily associated with defense response and protein phosphorylation;cellular component DEGs were enriched in the plasma membrane and membranes;molecular function DEGs were dominated by ATP binding and DNA-binding transcription factor activity.KEGG pathway analysis identified 14 significantly enriched pathways,including plant hormone signal transduction,plant-pathogen interaction,MAPK signaling pathway-plant and phenylpropanoid biosynthesis.[Conclusion]B.velezensis BS19 enhances tobacco resistance to bacterial wilt by modulating defense-related genes and key sig-naling pathways,which provides a theoretical foundation for the development of biocontrol agents.