Electrochemical immunosensor of graphene oxide/Au/Ag and strawberry-like Au@PtPd for exosome detectionOA
Early detection and treatment of colorectal cancer can effectively reduce the harm caused to patients.However,early symptoms are not obvious.Exosomes are extracellular vesicles that carry information between cells circulating in the human body.Despite the critical importance of tumor-derived exosomes for early cancer detection,prognosis,and treatment guidance,sensitive detection of exosomes remains challenging.We report a novel immunosensor for the detection of exosomes using graphene oxide(GO)/Au/Ag as the electrochemical sensing platform and spherical Au@PtPd porous nanoparticles to provide signal amplification.A large-surface-area GO/Au/Ag-modified screen-printed carbon electrode(SPCE)is used to immobilize CD63 aptamers.The prepared strawberry-like Au@PtPd demonstrates improved sensitivity and electrocatalytic activity with increasing aptamer load.Meanwhile,its good conductivity accelerates the electron transfer rate on the SPCE.The immunosensor exhibits a linear range(100–1.0×10^(6)exosomes·μl^(−1))and a detection limit of 23 exosomes·μl^(−1).Furthermore,its potential for the detection of exosomes provides a new clinical application for early diagnosis of colorectal tumors.
Rong Yang;Xiaorui Zhu;Guidan Wang;Jiling Shi;Xin Wang;Aihua Jing
School of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,ChinaCollaboration Innovative Center of Henan Province for Energy-Saving Building Materials,Xinyang Normal University,Xinyang 464000,ChinaSchool of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,ChinaSchool of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,ChinaSchool of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,ChinaSchool of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,China
医药卫生
Au@PtPdGraphene/Au/AgExosomesDetectionImmunosensor
《Nanotechnology and Precision Engineering》 2026 (1)
P.52-59,8
supported by National Natural Science Foundation of China(Grant No.12105239)the Science and Technology Projects of Henan Province(Grant No.232102310002).
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