Production of functional food Shuangshen tablets and their preventive effects on brain and liver injuries associated with D-galactose-induced aging in mice:an oxidative stress inhibition-based mechanismOA
Background:Aging is a key risk factor for human health,causing damage to the brain and liver tissues.Accumulating evidence indicates that oxidative stress is a critical driver of the aging process.Shuangshen tablet(SST),a functional food formulated with Ginseng Radix et Rhizoma and Apostichopus japonicus as main ingredients,possesses potential antioxidant and anti-aging properties.Thus,this study aimed to evaluate the anti-aging effects of SST using a D-galactose-induced aging model.Methods:The formula of SST was optimized via single-factor experiments and response surface methodology.Chemical components of SST were identified by UHPLC-Q-Exactive Orbitrap HRMS,and their in vitro antioxidant activity was determined.D-galactose-induced aging mice were intervened with SST,followed by detection of serum oxidative stress and pro-inflammatory factor levels,as well as the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)content in brain and liver tissues.Hematoxylin and eosin(HE)staining was used to observe brain and liver histopathological changes.Mechanistic studies were conducted to analyze the protein expression levels of Nrf2-HO-1 pathway-related proteins,phosphorylated PI3K(p-PI3K),and phosphorylated AKT(p-AKT).Acute and subacute toxicity tests were performed to evaluate the safety of SST.Results:A total of 46 active components were identified from SST,including ketones,terpenes,phenylpropanoids,and organic acids.SST intervention significantly regulated serum oxidative stress and pro-inflammatory factor levels in aging mice:it reduced MDA content in brain and liver tissues,increased SOD,CAT,and GSH-Px activities,and decreased serum tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β)levels.HE staining showed that SST alleviated D-galactose-induced brain and liver damage.Mechanistically,SST mitigated oxidative damage by activating the Nrf2-HO-1 pathway and upregulating the protein expression of p-PI3K and p-AKT.Acute and subacute toxicity tests confirmed the safety of SST.Conclusion:SST can effectively delay the D-galactose-induced aging process by improving the oxidative stress signaling pathway,providing a theoretical basis for the development of antioxidant functional foods.
Ru-Ping Xiao;Si-Wen Zheng;Hong-Yue Gao;Heng Wei;Ming Liang;Rui Zhang;Wan-Ying Li;He Yang;Nan-Qi Zhang;Ying-Ping Wang;Song-Yun Han
College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Agronomy and Biotechnology,Hebei Key Laboratory of Crop Stress Biology,Hebei Normal University of Science&Technology,Qinhuangdao 066600,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,ChinaR&D Department,Songyun Wild Economic Plants Co.,Ltd.,Baishan 134300,China
轻工纺织
functional foodantioxidantagingsubacute toxicity
《Traditional Medicine Research》 2026 (8)
P.28-42,15
funded by Jilin Provincial Science and Technology Development Plan Project-Research and Development of Shuangshen Tablets(20210401123YY)Jilin Provincial Science and Technology Development Plan Project-Jilin Province High-quality and Authentic Medicinal Materials(Ginseng)Science and Technology Demonstration Base+(Ji''an Dadi Ginseng Industry Co.,Ltd.Ginseng Planting Base)(20220401114YY)Scientific Research Fund of Hebei Normal University of Science&Technology(2024YB019).
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