Integrated analyses of metabolomics,transcriptomics,and network pharmacology reveal the effect of Shuangshi Tonglin Capsule against cervicitis in ratsOA
Background:Cervicitis is a common gynecological disease,which triggers local inflammation and tissue damage by a complex mechanism,seriously affecting women’s health.Chinese medicine plays a prominent role in the treatment of gynecological inflammation.Shuangshi Tongling Capsule(SSTL),as a traditional Chinese medicine(TCM)compound,needs to be further elucidated for the treatment of cervicitis.Methods:Cervicitis was induced with 25%phenol gel(except controls),followed by 12-day intragastric treatment.Outcomes included genital inflammation scores,uterine index,and histopathology(HE staining).Serum tumor necrosis factor-α(TNF-α),Interleukin(IL)-6,IL-8,and IL-1βwere measured via ELISA.Integrated metabolomics-transcriptomics and Western blotting identified mechanistic pathways.Results:SSTL alleviated cervicitis in rats by improving uterine histopathology,reducing external genital inflammation scores(P<0.01 vs.model),and reversing uterine hypertrophy(P<0.05).Serum pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-8)were suppressed(P<0.01),with concurrent weight restoration.Multi-omics analysis identified AMPK signaling as pivotal,validated by downregulation of p-NF-κB(P<0.05)and upregulation of p-AMPK/SIRT1(P<0.05)in uterine tissues,indicating SSTL mitigates inflammation through AMPK/NF-κB/SIRT1 modulation.Conclusion:This study found that SSTL ameliorates cervicitis in rats through the expression of AMPK pathway-related proteins.
Bo Han;Fei Luan;Xiao-Fei Zhang;Dong-Yan Guo;Bing-Tao Zhai;Shu-Guang Yan;Bo Wang;Bao-An Wang;Jun-Bo Zou;Ya-Jun Shi
Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaResearch and Development Department,Shaanxi Momentum Qixuehe Pharmaceutical Co.,Ltd,Xianyang 100016,ChinaResearch and Development Department,Shaanxi Momentum Qixuehe Pharmaceutical Co.,Ltd,Xianyang 100016,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,ChinaShaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,School of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China
医药卫生
SSTLcervicitismetabolomicstranscriptomicsnetwork pharmacology
《Traditional Medicine Research》 2026 (7)
P.25-36,12
supported by Science and Technology Innovative Talent Program of Shaanxi University of Chinese Medicine(No.2024-CXTD-03)Key Research&Development Program of Shaanxi Provincial Department of Science and Technology(No.2024CY-JJQ-36,2024CY-JJQ-78)Shaanxi Provincial Administration of Traditional Chinese Medicine Research and Innovation Team Project(No.TZKN-CXTD-03)Youth Innovation Team of Aromatic Chinese Medicine Industrialization Key Technology(No.2022-95)Scientific Research Project of Shaanxi Administration of Traditional Chinese Medicine(ZYJXG-L23002)the Science and Technology Innovative Team Project of Shaanxi Administration of Traditional Chinese Medicine(No.2025-CXTD-02)The cartoon elements in the mechanism diagram are from https://www.figdraw.com.Shaanxi Provincial Science and Technology Department’s“two chains”integrated enterprise(institute)joint project(2023-LL-QY-39).
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