Amplicon sequencing reveals the cryptic diversity in the dicyemid parasites of coleoid cephalopods sampled from the Atlantic and Pacific OceansOA
Dicyemids(phylum Dicyemida),primarily found in the renal organs of coleoid cephalopods,are a unique group of morphologically simple parasites with global distribution.Here,we investigated the diversity and prevalence of dicyemid communities in a wide range of cephalopod hosts across four geographic zones(the North East Atlantic,Mediterranean Sea,China Sea in the Western North Pacific,and Australia in the South Pacific)using Illumina sequencing of the 18S rDNA amplicons.Across 227 host samples,we identified 482 amplicon sequence variants,which clustered into 95 genetic types.The results indicated a higher number of distinct genetic types within Dicyemida than those currently identified through morphology-based taxonomy.Our finding of 46 dicyemid types in the common cuttlefish(Sepia officinalis)contrasts sharply with the previous records of a maximum of four species in this host.Furthermore,only a few host species exhibited a single dicyemid type,while most harbored multiple types;several types were distributed worldwide.Additionally,we identified eight new cephalopod hosts in the Pacific.Analyses of community(α)diversity suggested the unique character of certain geographical areas,such as the Bass Strait(Australia).β-diversity analyses confirmed that geographic location and host species were significant determinants of the dicyemid community composition.These results suggest that current species classifications may underestimate the true diversity of dicyemids.They emphasize the intricate interplay between geography,host specificity,and dicyemid community diversity.
Tijana Cvetković;Masoud Nazarizadeh;Tereza Koudelková;Fedor Lishchenko;Yen H.T.Dinh;Eduardo Almansa;Hannah Osland;Tomás Scholz;Zdeněk Lajbner;Qiaz Q.H.Hua;Marie Drábková;Jan Stefka
Institute of Parasitology,Biology Centre CAS,Česke Budějovice,37005,Czech Republic Department of Biological Sciences,University at Buffalo,Buffalo,NY,14260,USAInstitute of Parasitology,Biology Centre CAS,Česke Budějovice,37005,Czech Republic Faculty of Science,University of South Bohemia,Česke Budějovice,37005,Czech RepublicInstitute of Parasitology,Biology Centre CAS,Česke Budějovice,37005,Czech Republic Faculty of Science,University of South Bohemia,Česke Budějovice,37005,Czech RepublicA.N.Severtsov Institute of Ecology and Evolution of the RAS,Moscow,119071,Russia Coastal Branch of the Joint Vietnam—Russia Tropical Science and Technology Research Center,Nha Trang,650000,VietnamCoastal Branch of the Joint Vietnam—Russia Tropical Science and Technology Research Center,Nha Trang,650000,VietnamCentro Oceanográfico de Canarias,Instituto Español de Oceanografía(IEO),CSIC,Santa Cruz de Tenerife,38180,SpainCollege of Science and Technology,Temple University,Philadelphia,PA,19122,USAInstitute of Parasitology,Biology Centre CAS,Česke Budějovice,37005,Czech Republic Faculty of Science,University of South Bohemia,Česke Budějovice,37005,Czech RepublicPhysics and Biology Unit,Okinawa Institute of Science and Technology Graduate University,Okinawa,904-0495,JapanEnvironment Institute,Department of Ecology and Evolution,The University of Adelaide,Adelaide,SA,5005,AustraliaFaculty of Science,University of Hradec Králové,Hradec Králové,50003,Czech RepublicInstitute of Parasitology,Biology Centre CAS,Česke Budějovice,37005,Czech Republic Faculty of Science,University of South Bohemia,Česke Budějovice,37005,Czech Republic
生物科学
DicyemidaCephalopodsHost–parasite assemblageAmplicon sequencingBiodiversity
《Marine Life Science & Technology》 2026 (1)
P.16-31,16
Computational resources were provided by the e-INFRA CZ project(ID:90254),supported by the Ministry of Education,Youth and Sports of the Czech Republicfunded by the Czech Science Agency(Project No.19-28399X).
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