3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscopeOA
Wide-field quantum sensing with fluorescent nanodiamonds(FNDs)in biological systems offers significant potential for understanding intracellular dynamics at the nanoscale.However,current wide-field quantum sensing methods are limited to 2D correlated measurements.3D correlated quantum sensing remains challenging due to the inherent properties of wide-field microscopy.Here,we have developed a multi-plane wide-field microscope platform that achieves an imaging volume of 50×50×5μm^(3).This is accomplished by simultaneously imaging eight focal planes at varying sample depths using a beam-splitting prism.By employing a Fourier-transform-based fluorescent particle positioning method,the platform attains lateral positioning precision of 9 nm and axial precision of 12 nm.Using this platform,we performed correlated 3D positioning of FNDs in mouse cardiomyocytes and conducted optically detected magnetic resonance on nitrogen-vacancy color centers within intracellular FNDs.Our results demonstrate the potential of this platform for single-particle tracking and highlight its capability to achieve correlated 3D quantum sensing.
Bowen Zhang;Yichen Yang;Jia Su;Linyu Zeng;Zenghao Kong;Zhijie Li;Zhiping Yang;Fazhan Shi
CAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,China School of Biomedical Engineering and Suzhou Institute for Advanced Research,University of Science and Technology of China,Suzhou 215123,ChinaCAS Key Laboratory of Microscale Magnetic Resonance and School of Physical Sciences,University of Science and Technology of China,Hefei 230026,China Anhui Province Key Laboratory of Scientific Instrument Development and Application,University of Science and Technology of China,Hefei 230026,China Hefei National Laboratory,University of Science and Technology of China,Hefei 230088,China School of Biomedical Engineering and Suzhou Institute for Advanced Research,University of Science and Technology of China,Suzhou 215123,China
机械制造
Multi-plane microscopeFluorescent nanodiamondsOptically detected magnetic resonanceNitrogen-vacancy centerSingle-particle trackingCorrelated quantum sensing
《Biophysics Reports》 2026 (1)
P.8-17,10
supported by the National Natural Science Foundation of China(T2125011),the CAS(YSBR-068)the Innovation Program for Quantum Science and Technology(2021ZD0302200,2021ZD0303204),New Cornerstone Science Foundation through the XPLORER PRIZEthe Fundamental Research Funds for the Central Universities.
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