Technological innovations and high-throughput applications of light-sheet microscopyOA

Light-sheet fluorescence microscopy(LSFM),with its innovative design of selective plane illumination and orthogonal detection optics,significantly reduces phototoxicity and photobleaching inherent in conventional microscopy,providing a revolutionary tool for long-term dynamic imaging of living specimens.This review focuses on throughput enhancement strategies of LSFM,systematically summarizing advancements in optical architecture optimization and multimodal integration.Key technological innovations include:improved sample compatibility,large-field imaging via optimized light-sheet generation,microfluidics-coupled high-throughput automation,and hyperspectral imaging for multiplexed analysis.Through adaptive light-sheet modulation,remote focusing synchronization,and AI-driven algorithmic optimization,LSFM achieves multiscale 3D imaging spanning subcellular structures to centimeter-scale tissues at speeds exceeding hundreds of volumetric frames per second.In biomedical applications,LSFM has successfully resolved complex processes such as cellular lineage dynamics during embryogenesis,whole-brain neuronal activity mapping,and structure-function correlations in cardiovascular systems,while enabling high-throughput drug screening and pathological model analysis.These breakthroughs establish LSFM as a cornerstone technology for intravital imaging,offering an integrated solution that combines high spatiotemporal resolution,minimal photodamage,and big-data throughput.By bridging molecular,cellular,and organ-level observations,LSFM drives paradigm shifts in developmental biology,neuroscience,and translational medicine,empowering unprecedented exploration of living systems across scales.