Hua-Yi-Jie-Du formula ameliorates poly(I:C)-induced viral pneumonia by regulating ILC2 cells and NLRP3 activationOA
Background:Hua-Yi-Jie-Du formula(HYJD)is a traditional Chinese medicine that has proven effective against viral pneumonia and was extensively used during the COVID-19 pandemic.This study investigates how HYJD influences group 2 innate lymphoid cell(ILC2)and nucleotide oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation in a mouse model of viral pneumonia.Methods:A mouse model of viral pneumonia was established through the administration of polyinosinic-polycytidylic acid(poly(I:C))via nasal drops.Histopathological analysis of lung tissue was conducted,alongside enzyme-linked immunosorbent assay to quantify cytokine levels in serum and bronchoalveolar lavage fluid(BALF).Flow cytometry was employed to detect ILC2 cells in lung tissue and spleen,while immunofluorescence techniques were utilized to visualize ILC2 cells in lung tissue.Transcriptomic sequencing was performed,and the results were validated using qRT-PCR and western blot analysis.Results:HYJD significantly ameliorated inflammatory infiltration in lung tissue,decreased mucus protein secretion,and reduced the serum levels of inflammatory cytokines interleukin(IL)-1β,IL-6,and tumor necrosis factor-alpha(TNF-α).Additionally,it lowered the expression of cytokines IL-4,IL-5,IL-13,IL-25,thymic stromal lymphopoietin(TSLP),and IL-33 in BALF,and reduced the differentiation of ILC2 cells in both lung tissue and spleen.Transcriptomic analysis and experimental validation revealed that HYJD downregulated the expression of NLRP3 related genes and proteins within the NOD-like receptor signaling pathway.Conclusion:The mechanism by which HYJD intervenes in acute lung injury associated with viral pneumonia may involve the reduction of ILC2 cells differentiation and the inhibition of NLRP3 activation.
Xiao-Hong Li;Ning Yang;Chun-Fei Zhang;Die Qian;Guo-Hui Yang;Xin-Yan Yang;Chun-Rong He;Zhi-Dao Zhang;Bi-Hong Dai;Chun-Ping Wan
Department of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,China School of Clinical Medicine,Yunnan University of Chinese Medicine,Kunming 650500,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaSchool of Clinical Medicine,Yunnan University of Chinese Medicine,Kunming 650500,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,China School of Clinical Medicine,Yunnan University of Chinese Medicine,Kunming 650500,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,ChinaDepartment of Scientific Research,The Fifth Affiliated Hospital,Yunnan University of Chinese Medicine,Chuxiong 675000,China School of Clinical Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China
医药卫生
viral pneumoniaHua-Yi-Jie-Du formulaILC2 cellsNLRP3inflammatory response
《Traditional Medicine Research》 2026 (6)
P.50-59,10
supported by Yunnan Provincial Major Science and Technology Special Program(No.202402AA310035)Yunnan Key Laboratory of Dai and Yi Medicines(Yunnan University of Chinese Medicine)(No.2024JS2404)Research Foundation of Chuxiong Medical College(No.2024YYXM38).
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