3 种嘌呤生物碱在卷烟烟气中转移行为研究OA

[Objective]To investigate the pyrolysis characteristics of three purine alkaloids and their transfer behaviors in cigarette smoke.[Methods]The pyrolysis behaviors of theobromine,theophylline,and caffeine were investigated by pyrolysis-gas chromatography/mass spectrometry(Py-GC/MS)hyphenation.A semi-quantitative analytical method using ultra-high performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS)was established for the semi-quantitative determination of the three alkaloids in mainstream cigarette smoke,and the pretreatment conditions were optimized to determine the transfer rates of the alkaloids in mainstream cigarette smoke.[Results]In the pyrolysis temperature range of 300℃and 600℃,the three alkaloids were predominantly transferred in their original form;however,when the pyrolysis temperature increased to 900℃,their pyrolysis extent was significantly enhanced and their contents decreased markedly,and no polycyclic aromatic hydrocarbons(PAHs)were generated during pyrolysis.The highest spiked recoveries of the three alkaloids were obtained under the following optimized conditions:extraction solvent:NaOH solution(pH value 11.9),extraction method:reflux extraction,extraction time:4.0 h,and detection method:UPLC-HRMS,which met the technical requirements of semi-quantitative analysis.The transfer rates of theobromine,theophylline,and caffeine in mainstream cigarette smoke were 0.21%,0.74%,and 0.10%,respectively,with theophylline having the highest transfer rate.[Conclusion]Based on the high-temperature transfer characteristics of the three purine alkaloids,an analytical technology system for trace alkaloids in complex tobacco matrices was constructed.This system can provide a reference for the quality control of tobacco products,their health risk assessment,as well as the study on the smoke transfer behaviors of high-boiling-point flavor components.