基于丝裂原活化蛋白激酶信号通路探讨黄芪多糖对肠上皮-巨噬细胞共培养肠炎模型的改善作用及机制OA

Objective:To investigate the ameliorative effect of astragalus polysaccharide(APS)on the inflammatory response in the Caco-2/RAW264.7 co-culture intestinal model and explore its potential mechanism.Methods:Different concentrations of APS(50 μg/mL,100 μg/mL)were applied to the Caco-2/RAW264.7 co-culture intestinal inflammation model induced by Lipopolysaccharide(LPS).The protective effect of APS on intestinal barrier function damage was evaluated by measuring the activities of intestinal alkaline phosphatase(IAP)and diamine oxidase(DAO),and the expression of tight junction protein zonula occludens-1(ZO-1).Meanwhile,the potential molecular mechanism of APS in improving intestinal inflammation was explored by determining the levels of inflammatory cytokines(TNF-α,IL-1β,IL-6)and the phosphorylation levels of ERK and p38 MAPK proteins in the mitogen-activated protein kinase(MAPK)signaling pathway.Results:Compared with the model group,the activities of IAP and DAO in the high-dose group(100 μg/mL)were statistically significantly increased(P<0.05).The expression of ZO-1 protein in both the high-dose and low-dose groups(100 μg/mL,50 μg/mL)were statistically significantly increased in a dose-dependent manner(P<0.05).In addition,the secretion of inflammatory cytokines TNF-α,IL-1β,and IL-6 in the high-dose group were statistically significantly reduced(P<0.05).Both the high-dose and low-dose groups significantly inhibited the activation of the MAPK signaling pathway and decreased the phosphorylation levels of ERK and p38 MAPK proteins,thereby effectively suppressing the inflammatory response(P<0.05).Conclusion:APS can alleviate the inflammatory response in the intestinal co-culture model by inhibiting the MAPK signaling pathway and protect the integrity of the intestinal barrier,which provides a theoretical basis for the application of APS in the development of natural anti-inflammatory foods and drugs.