巴氏杀菌对枯草芽孢杆菌芽孢灭活效果的影响OA

[Objective]To improve the efficiency of pasteurization on the spore inactivation of Bacillus subtilis 168.[Methods]The changes in spore count and sporulation rate of B.subtilis 168 under different pasteurization conditions were determined,and the spore survival rate after synergistic treatment of pasteurization with different sanitizers(H2 O2,nitrite,glutaraldehyde,povidone-iodine)was also measured to analyze the spore inactivation efficiency.[Results]When B.subtilis 168 was subjected to pasteurization at 95℃for 15 min at the late logarithmic growth phase(8 h of culture),it exhibited the best spore inactivation effect,with the final spore count and sporulation rate being 7 log CFU/mL and 1%,respectively.In addition,although this treatment did not completely inhibit the proliferative activity of residual bacterial cells,it significantly reduced their spore-forming ability.Compared with pasteurization alone,there was no significant change in the spore survival rate of B.subtilis 168 when pasteurization was combined with H2 O2.However,the spore survival rate of B.subtilis 168 was significantly reduced when pasteurization was combined with nitrite,glutaraldehyde,or povidone-iodine,respectively.Among them,the combination of pasteurization with nitrite or glutaraldehyde achieved a significant spore inactivation effect within a short time(90 min),with the spore survival rate being approximately 20%～30%,while it required 4 h of synergistic treatment with povidone-iodine to achieve a similar inactivation effect.[Conclusion]Both nitrite and glutaraldehyde can significantly improve pasteurization's effectiveness in the spore inactivation of B.subtilis 168.