印度榕白绢病病原菌的分离鉴定及室内药剂筛选OA

To identify the pathogenic fungi responsible for southern blight in Ficus elastica and to identify suitable control agents,the pathogen was isolated and identified using a combination of morphological and molecular biological methods,and fungicides were screened in vitro using the toxic medium method.Based on pathogenicity tests,morphological observations and rDNA-ITS sequence homology analysis,the pathogen causing the southern blight in Ficus elastica was identified as Agroathelia rolfsii.The results of indoor fungicide screening showed that difenoconazole,fludioxonil,and pyraclostrobin exhibited the strongest inhibitory effects against the tested strain,with median effective concentration(EC50)values of 0.07,0.08 μg/mL and 0.15 μg/mL,respectively three days after treatment.This was followed by azoxystrobin,picoxystrobin,and prochloraz,with EC50 values of 2.09,3.96 μg/mL and 15.72 μg/mL,respectively.Carbendazim showed the lowest inhibitory effect,with an EC50 value of 604.04 μg/mL.This study confirmed that A.rolfsii is the pathogen for southern blight in Ficus elastica,and that difenoconazole,fludioxonil,and pyraclostrobin have strong inhibitory effects against this pathogen and can be recommended as effective control agents.