我国首次发现由Alternaria arborescens引起的甘薯叶柄和茎秆枯萎病OA
First report of leaf petiole and stem blight of sweetpotato caused by Alternaria arborescens in China
2021年—2023年从山东、云南等地采集在叶片、叶柄及茎秆等部位产生椭圆形病斑的甘薯样品,通过组织分离法获得病原菌,标为Z4hei和YN2,采用形态学和分子生物学技术对其鉴定.结果表明,在PDA培养基上2个菌株菌落初为白色后变为墨绿色至黑色.分生孢子椭圆形或豆荚状,具1～6个横隔,0～2个纵隔.2个菌株的rD-NA-ITS序列长度均为569 bp,一致性为100％,与NCBI上交链格孢Alternaria alternata(MK370642)和枝状链格孢A.arboresscens(PP097783)一致性最高,为100％.为进一步确定病原菌种类,分别扩增菌株的RPB2与TEF1-α基因,2个菌株RPB2长度均为985 bp,一致性为100％,与A.arboresscens(XM_028644303)一致性最高,为99.59％.2 个菌株的 TEF1-α 基因序列长度均为 280 bp,一致性为 99.60％,与 NCBI 上 A.arborescens(OK081882)一致性最高,分别为99.29％和98.93％.由此证实引起我国甘薯叶柄和茎秆枯萎病的病原菌是A.arborescens,为该病在我国发生的首次报道,本研究为其防治提供了理论依据.
From 2021 to 2023,sweetpotato samples exhibiting elliptical lesions on leaves,petioles,and stems were collected from Shandong and Yunnan provinces,China.To identify causal agent,pathogens were isolated from diseased tissues and designated as strains Z4hei and YN2,which were identified using morphological and molecular methods.The colonies of both strains were initially white on potato dextrose agar(PDA)and gradually turned dark green to black.Conidia were elliptical or bean-shaped,with 1-6 transverse septa and 0-2 longitudinal septa.The rDNA-ITS sequences of both strains were 569 bp in length and showed 100％identity with each other,exhibiting the highest similarity(100％)with Alternaria alternata(MK370642)and A.arborescens(PP097783)in the NCBI database.To further confirm species identification,the RPB2 and TEF1-α genes were amplified and sequenced.The RPB2 sequences were 985 bp in length and showed 100％identity between the two strains,with 99.59％similarity to A.arborescens(XM_028644303).The TEF1-α sequences were 280 bp in length,sharing 99.60％identity between the strains and showing the highest similarity to A.arborescens(OK081882),with values of 99.29％and 98.93％,respectively.Based on morphological and molecular analyses of rDNA-ITS,RPB2,and TEF1-α sequences,the pathogen causing leaf petiole and stem blight of sweetpotato in China was identified as A.arborescens.This study represents the first report of A.arborescens causing this disease in China.This study provides a theoretical basis for prevention and control of leaf petiole and stem blight of sweetpotato.
张成玲;孙厚俊;唐伟;马居奎;董谢楠;陈晶伟;高方园;杨冬静;梁昭;佟聪
江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,徐州 221131
农业科技
甘薯叶柄和茎秆枯萎病枝状链格孢病原菌鉴定
sweetpotatoleaf petiole and stem blightAlternaria arborescenspathogen identification
《植物保护》 2026 (2)
206-211,6
国家甘薯产业技术体系(CARS-10)
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