基于血清代谢组学研究厚朴炮制前后对急性肺损伤大鼠模型的作用机制OA

Objective:To investigate the therapeutic effect and mechanism of Magnolia Officinalis Cortex(Magnolia offici-nalis)before and after processing on acute lung injury(ALI)using serum metabolomics technology.Methods:Forty SD rats were randomly divided into a blank control group(Group K),model group(Group M),crude Magnolia officinali group(Group S),and a ginger-processed Magnolia officinalis group(Group A),with 10 rats per group.Groups S and A were administered the corre-sponding drug solutions by gavage daily for 7 days.The ALI model was established via intraperitoneal lipopolysaccharide(LPS)injection after the last administration.Lung injury was assessed by hematoxylin and eosin(H&E)staining and the lung wet/dry weight ratio(W/D).Serum levels of inflammatory factors(IL-6,IL-1β,TNF-α)and activities of oxidative stress indicators(SOD,MPO)were measured using enzyme-linked immunosorbent assay(ELISA).Liquid chromatography-mass spectrometry was em-ployed for serum metabolomics analysis across all groups to identify potential differential metabolites,followed by metabolic path-way analysis.Results:Compared with Group M,both Groups S and A showed alleviated lung histopathological damage and re-duced W/D ratio,with Group A demonstrating more significant improvement than Group S(P＜0.05).Regarding the inhibition of inflammation and oxidative stress,Group A exhibited superior regulatory effects on IL-1β(P＜0.01),TNF-α,and MPO(P＜0.05)compared to Group S.Metabolomics analysis identified 102 differential metabolites callback-regulated by ginger-processed Mag-nolia officinalis(compared to 83 for the crude products),primarily involved in metabolic pathways such as beta-alanine metabo-lism,tricarboxylic acid(TCA)cycle,and histidine metabolism.Conclusion:The therapeutic effect of Magnolia officinalis again st ALI is enhanced after processing with ginger.Building on the metabolic regulatory characteristics of the raw product,it further expands the regulatory scope of endogenous metabolites and differentially modulates core pathways,including protein digestion and absorption,central carbon metabolism,and sphingolipid signaling.