冬虫夏草多糖提取方法比较与优化OA

Objective:To improve the extraction yield of Cordyceps polysaccharides and optimize the extraction process of Cordyceps polysaccharides.Methods:Cordyceps polysaccharides were extracted using ultrasound-assisted extraction and internal boiling method,and the effects of these two methods on polysaccharide yield were compared through single-factor experiments.The optimal method was selected for further Box-Behnken response surface methodology to optimize the extraction process.Re-sults:The ultrasonic-assisted extraction method outperformed the internal boiling method,with extraction rates of 2.718％and 2.058％,respectively.Through response surface analysis,the optimal extraction conditions for Cordyceps polysaccharides were determined as follows:ultrasonic temperature set at 55℃,ultrasonic power adjusted to 280 W,ultrasonic treatment time of 30 min,and a liquid-to-material ratio of 30.Three replicate experiments were conducted under these conditions to verify the reliabil-ity of the optimized process,resulting in an average polysaccharide extraction yield of 2.714％.Conclusion:The optimized extrac-tion process is efficient,time-saving,and conducive to the further development and utilization of natural Cordyceps.