灸药联合对三阴性乳腺癌小鼠肿瘤组织抗原提呈细胞CD80及CD86表达的影响OA

Objective:To observe the effect of moxibustion combined with chemotherapy on the expression of costimulatory molecules CD80 and CD86 on antigen-presenting cells(APCs)in tumor tissues of mice with triple-negative breast cancer(TNBC),and to explore the intervention effect of direct moxibustion combined with chemotherapeutic drugs on TNBC. Methods:Fifty BALB/c mice were divided into a normal group,a model group,a medication group,a direct moxibustion group,and a moxibustion plus medication group using the random number table method,with 10 mice in each group.The normal group was not given any intervention,and the other four groups were injected with 4T1 cells under the left fourth mammary fat pad to prepare tumor-bearing mouse models.One week after modeling,the direct moxibustion group received direct moxibustion at Zusanli(ST36);the medication group received intraperitoneal injection of doxorubicin;the moxibustion plus medication group received direct moxibustion at Zusanli(ST36)and intraperitoneal injection of doxorubicin together,all for 21 d.After the intervention finished,the mouse's tumor volume in each group was measured;hematoxylin-eosin staining was used to observe the morphology of tumor tissues;immunohistochemistry and Western blotting were used to detect the expression of CD80 and CD86 in tumor tissues.The positive expression of CD80 in tumor tissues and CD4+,CD8+,and CD4+/CD8+in peripheral blood was detected by flow cytometry. Results:CD4+/CD8+in the peripheral blood in the other groups were decreased compared to the normal group(P<0.01).Tumor volumes were decreased(P<0.01),cell morphology was abnormal or fragmented,cell arrangement was disordered,the expression of CD80 and CD86 was increased to different degrees(P<0.01 or P<0.05),and CD4+/CD8+was increased(P<0.01)in tumor cells in the other groups compared to the model group.Compared to the medication group,the expression levels of CD80 and CD86(P<0.01 or P<0.05),as well as CD4+/CD8+(P<0.01)were decreased in the direct moxibustion group,and the improvement effect on the above indicators in the moxibustion plus medication group was more significant(P<0.01 or P<0.05). Conclusion:Compared to the chemotherapeutic drug or direct moxibustion alone,the combination of moxibustion with the chemotherapeutic drug has a better intervention effect on TNBC mice,especially in inhibiting tumor growth,upregulating the expression of costimulatory molecules CD80 and CD86 on APCs,and promoting the restoration of T lymphocyte subsets to balance.