泰山磐石散加减方体外抗氧化活性评价OA

To evaluate the antioxidant activity of the modified Taishan Panshi San formula in vitro,four classical models(DPPH·,ABTS+·,·OH and FRAP)were employed,with vitamin C(VC)acting as the positive control,to determine its capacity to scavenge DPPH·,ABTS+·,·OH radicals and reduce Fe3+(FRAP assay).Absorbance was measured at 517 nm,734 nm,520 nm and 593 nm,respectively,and the scavenging rates were calculated.Fe3+reduction capacity was determined via a FeSO4 standard curve.The results showed that the scavenging rates of the modified Taishan Panshi San formula against all three free radicals increased with concentration,exhibiting a clear dose-response relationship.The IC50 values were 0.274 5 mg/mL for DPPH·radicals,0.153 1 mg/mL for ABTS+·radicals and 0.526 7 mg/mL for·OH,all of which were lower than the IC50 value of vitamin C(0.002 1 mg/mL,0.011 3 mg/mL and 0.024 9 mg/mL,respectively).The Fe3+reduction capacity of the modified Taishan Panshi San formula exhibited a positive correlation,increasing with formula concentration,while Fe3+reduction capacity of the formulation(0.059 5 mmol FeSO4/g)was significantly lower than that of vitamin C(100.830 8 mmol FeSO4/g).Taishan Panshi San demonstrates scavenging activity against DPPH·,ABTS+·and hydroxyl radicals,as well as iron ion reduction capacity.