基于网络药理学和实验验证探讨"黄芪-丹参-三七"角药对心衰大鼠氧化应激的影响OA

Aim To investigate the effects of Astragalus-Danshen-Sanqi horn medicine on oxidative stress in rats with heart failure model based on network pharmacology and experimental validation.Methods The drug active components,core targets and signal-ing pathways of Astragalus-Danshen-Sanqi horn medi-cine against oxidative stress in heart failure were screened by network pharmacology,and the core tar-gets were molecularly docked with the drug active com-ponents.Forty SD rats were randomly divided into four groups,the control group,model group,horn medi-cine group and western medicine group,and the corre-sponding drug interventions were given according to the subgroups.The changes in the level of cardiac function indexes in each group were compared using cardiac ultrasound,the pathological changes in car-diac tissues in each group were evaluated using HE staining,and the fibrosis of cardiomyocytes in each group was assessed using Masson staining;the changes in the ultrastructure of mitochondria of cardio-myocytes in each group were observed using transmis-sion electron microscopy;and the changes in the level of oxidative stress indexes in the cardiac tissues of rats in each group were compared.Results The key phar-macological components screened were quercetin,kaempferol,and lignocerotoxin,and the core targets of action were TNF,IL6,IL1B,TP53,AKT,and CASP3.Animal experiments showed that the cardiac function of rats was significantly improved when com-pared with that of the model group,and the results of HE and Masson staining showed that the structural damage of myocardial tissues was attenuated,the car-diomyocytes were less fibrous.Electron microscopy re-vealed reduced mitochondrial structure damage,while ROS levels in myocardial tissue were reduced,and CAT and T-AOC levels increased(P<0.05).Conclu-sion Horn medicine is able to exert a protective ef-fect against heart failure by reducing oxidative stress,mitigating mitochondrial structural and functional dam-age,and promoting the restoration of cardiac systolic-diastolic functions.